Myelodysplastic Syndrome (MDS) — FISH Analysis

CPT Code(s):  88237, 88275, 88271 (x per probe)

Service Code (IU Health): 53101259, 53100707, 53100640

Ordering Recommendation: FISH is useful to (1) detect a neoplastic clone that is associated with common chromosome abnormalities observed in patients with MDS; (2) evaluate specimens in which standard cytogenetic analysis has been unsuccessful; and (3) identify and track chromosome abnormalities in patients with myeloid malignancies, including follow-up of patients to evaluate response to therapy.

Synonyms: FISH, hematopoietic malignancy, fluorescence in situ hybridization

Methodology: Fluorescence in situ hybridization (FISH) analysis.

Performed: Monday through Friday

Reported: 2-3 days

Panels: MDS Panel - 5q, 7q, CEP 8/20q, KMT2A/MLL (11q23) Each probe may also be ordered individually.

Collect: Non-diluted bone marrow aspirate in green-top sodium heparin tube, heparinized syringe.

Specimen Volume: Preferred: 2 mL for normal WBC (decreased WBC requires more, and elevated WBC requires less); Minimum: 0.5 mL

Storage/Transport: Room temperature. Do not freeze or expose to extreme temperatures.

Unacceptable Conditions: Frozen specimens. Formalin-fixed specimens.

Stability: Ambient: 48 hours; Frozen: Unacceptable