Solid Tumors, Paraffin-Embedded Tissue (PET) — FISH Analysis

CPT Code(s):  88275, 88271 (x per probe)

Service Code (IU Health): 53100707, 53100640

Ordering Recommendation: For detection of a neoplastic clone that is observed in patients with lymphoma or other solid tumors such as neuroblastoma and sarcoma, to identify and track chromosome abnormalities in patients, and to evaluate response to therapy.

Synonyms: FISH, hematopoietic malignancy, lymphoma, Hodgkin’s lymphoma, non- Hodgkin’s lymphoma, Burkitt Lymphoma, Burkitt-like Lymphoma, Diffuse Large Cell Lymphoma, Follicular Lymphoma, MALT Lymphoma, Mantle Cell Lymphoma, MYC - 8q24 Rearrangement, Neuroblastoma, Ewing sarcoma, MYCN, EWSR1

Methodology: Fluorescence in situ hybridization (FISH) analysis.

Performed: Tuesday, Thursday, Friday

Reported: 2-3 days

Panels: Lymphoma Panel - t(8;14)(MYC/IGH), MYC, t(14;18) (IGH/BCL2)

Collect: 10% buffered formalin-fixed, paraffin-embedded tissue (PET) specimen.

Specimen Volume: 4-micron sections on 2-3 positively charged slides with corresponding H&E section with area of tumor marked by pathologist.

Storage/Transport: Room temperature or refrigerated. Protect from excessive heat.

Unacceptable Conditions: Do not send a block. No decalcified bone. No specimens fixed/processed in non-10% buffered formalin. No frozen specimens.

Stability: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable